Publications

Please see the guidelines page for instructions on acknowledging us with our RRID:SCR_021114.

a wordcloud in the shape of an Erlenmeyer. Prominent words include Lokey, Rubin, clock.
source: wordclouds.com using entries 57-67.

(last updated 10 Dec 2024)

69. Liang CE, Hrabeta-Robinson E, Behera A, Arevalo C, Fetter IJ, Soulette CM, Thornton AM, Sikandar SS, Brooks AN. U2AF1 S34F enhances tumorigenic potential of lung cells by exhibiting synergy with KRAS mutation and altering response to environmental stress. bioRxiv [Preprint]. (2024) Sep 15:2024.09.11.612492. doi: 10.1101/2024.09.11.612492. PMID: 39314447

  • Imaging studies of cell cycle mutants used CSC BioTek bulk dispenser and washer, Phenix, and Harmony image analysis (figure 3B,C).
  • Cindy Liang, Eva Hrabeta-Robinson, and Isobel Fetter are trained users of the CSC. Cindy earned their PhD with this project in June 2024.

box plots show cell cycle markers (EdU and pHH3) for different mutant cell lines.

68. Baumgartner JT, McCaughey CS, Fleming HS, Lentz AR, Sanchez LM, McKinnie SMK. Vanadium-dependent haloperoxidases from diverse microbes halogenate exogenous alkyl quinolone quorum sensing signals. (2024) bioRxiv [Preprint]. 2024 Aug 1:2024.07.31.606109. doi: 10.1101/2024.07.31.606109. PMID: 39131370

  • OD600 of bacteria on CSC EnVision plate reader (figure 5)
  • PhD candidates Jackson Baumgartner and Adam Lentz are trained users of the CSC.
  • Beverley Rabbitts (CSC manager) is acknowledged.

growth curves over time for different bacterial cultures.

67. Rodríguez SG, Crosby P, Hansen LL, Grünewald E, Beale AD, Spangler RK, Rabbitts BM, Partch CL, Stangherlin A, O’Neill JS, van Ooijen G. Potassium rhythms couple the circadian clock to the cell cycle. bioRxiv [Preprint]. (2024) Apr 3:2024.04.02.587153. doi: 10.1101/2024.04.02.587153.
PMID: 38617352

  • Long timecourse live cell imaging on CSC Phenix and image analysis in Harmony (figure 5)
  • by Priya Crosby, a trained user of the CSC, during her postdoctoral training. She is now an independent investigator at U of Edinburgh.
  • Beverley Rabbitts (CSC manager) is an author.

graphs show oscillations for individual cells undergoing different treatments, and the period increases for increased dosing. A clock diagram shows a summary of potassium concentration at different cell cycle stages.

66. Barrett AK, Shingare MR, Rechtsteiner A, Rodriguez KM, Le QN, Wijeratne TU, Mitchell CE, Membreno MW, Rubin SM, Müller GA. HDAC activity is dispensable for repression of cell-cycle genes by DREAM and E2F:RB complexes. Nat Commun. (2024) May 24;15(1):4450. doi: 10.1038/s41467-024-48724-0. PMID: 38789411

  • HDAC activity assays using CSC EnVision plate reader (figures 3 and 4)
  • CSC trained users include Alison Barrett, Kelsie Rodriguez, Tilini Wijeratne, Miles Membreno, Gerd Müller. After earning her PhD with this project, Alison accepted a postdoctoral fellowship at Stanford.

graphs show HDAC activity for various substrates.
65. Lau TA, Mair E, Rabbitts BM, Lohith A, Lokey RS. High-Content Image-Based Screening and Deep Learning for the Detection of Anti-Inflammatory Drug Leads. Chembiochem. (2024) Jan 15;25(2):e202300136. doi: 10.1002/cbic.202300136. Epub 2023 Nov 7. PMID: 37815526

  • Cytological profiling, image-based assays, image quantification softwares, and cytokine sample prep conducted at the CSC using the Janus robot, Echo acoustic dispenser, various bulk dispensers, BioTek plate washer, ImageXpress and Phenix imagers, and bioinformatics with MetaXpress and Harmony (figures 1-4)
  • CSC Selleck Chem and TargetMol compound libraries. We also store the natural product libraries reported here.
  • Tannia Lau and Akshar Lohith are trained users of the CSC. After earning her PhD with this project, Tannia accepted a position as research scientist at Eli Lilly & Co.
  • Beverley Rabbitts (CSC manager) is a co-first author.
a colorful workflow showing cells being treated with compounds and LPS, then stained and imaged, machine learning to ID hits, followed by further experiments to validate hits.

64. Hight SK, Clark TN, Kurita KL, McMillan EA, Bray W, Shaikh AF, Khadilkar A, Haeckl FPJ, Carnevale-Neto F, La S, Lohith A, Vaden RM, Lee J, Wei S, Lokey RS, White MA, Linington RG, MacMillan JB. High-throughput functional annotation of natural products by integrated activity profiling. Proc Natl Acad Sci U S A. (2022) Dec 6;119(49):e2208458119. doi: 10.1073/pnas.2208458119. Epub 2022 Nov 30. PMID: 36449542

  • Cytological profiling (CSC Janus robot, bulk dispenser, BioTek plate washer, and ImageXpress – figures 1-5)
  • CSC Selleck Chem compound library. We also store the MacMillan and Linington natural product libraries reported here.
  • CSC trained users include Anam Shaikh, Aswad Khadilkar, Scott La, and Akshar Lohith.
  • Former CSC manager, Walter Bray was a coauthor.

a workflow diagram showing gene expression, cell imaging, and mass spectrometry all coming together.

63. Wijeratne TU, Guiley KZ, Lee HW, Müller GA, Rubin SM. Cyclin-dependent kinase-mediated phosphorylation and the negative regulatory domain of transcription factor B-Myb modulate its DNA binding. J Biol Chem. (2022) Sep;298(9):102319. doi: 10.1016/j.jbc.2022.102319. Epub 2022 Aug 1. PMID: 35926712

  • Fluorescence polarization on CSC EnVision plate reader (figures 1 & 4)
  • Ultrasensitive luminescence on CSC EnVision plate reader (figure 5)
  • Tilini Wijeratne and Gerd Müller are trained users of the CSC. After earning her PhD with this project, Tilini accepted a postdoctoral fellowship at Stanford.

a figure showing the protein structure in two conformations, which are low or high luminescence, and a graph of the luminescence plus westerns for different mutants

62. Swan JA, Sandate CR, Chavan AG, Freeberg AM, Etwaru D, Ernst DC, Palacios JG, Golden SS, LiWang A, Lander GC, Partch CL. Coupling of distant ATPase domains in the circadian clock protein KaiC. Nat Struct Mol Biol. (2022) Aug;29(8):759-766. doi: 10.1038/s41594-022-00803-w. Epub 2022 Jul 21. PMID: 35864165.

  • High throughput liquid handling (CSC Janus robot)
  • Plate reader (CSC EnVision)

61. Asthana A, Ramanan P, Hirschi A, Guiley KZ, Wijeratne TU, Shelansky R, Doody MJ, Narasimhan H, Boeger H, Tripathi S, Müller GA, Rubin SM. The MuvB complex binds and stabilizes nucleosomes downstream of the transcription start site of cell-cycle dependent genes. Nat Commun. (2022) Jan 26;13(1):526. doi: 10.1038/s41467-022-28094-1. PMID: 35082292.

  • Fluorescence polarization on CSC EnVision plate reader (figure 4)
  • CSC trained users include first author Anu Asthana, Tilini Wijeratne, and Gerd Müller. After earning her PhD with this project, Anu accepted a postdoctoral fellowship at Stanford.

a figure showing FPol graph

60. Enrico TP, Stallaert W, Wick ET, Ngoi P, Wang X, Rubin SM, Brown NG, Purvis JE, Emanuele MJ. Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2. Elife. (2021) Dec 1;10:e70691. doi: 10.7554/eLife.70691. PMID: 34851822 .

  • Fluorescence polarization on CSC EnVision plate reader (figure 4)

59. Klein VG, Bray WM, Wang HY, Edmondson Q, Schwochert J, Ono S, Naylor MR, Turmon AC, Faris JH, Okada O, Taunton J, Lokey RS. Identifying the Cellular Target of Cordyheptapeptide A and Synthetic Derivatives. ACS Chem Biol. (2021) Aug 20;16(8):1354-1364. doi: 10.1021/acschembio.1c00094. Epub 2021 Jul 12. PMID: 34251165 .

  • Luminescence for cytotoxicity on CSC EnVision plate reader (figures 1, 5)
  • Cytological profiling and image-based DNA synthesis studies used the CSC Janus robot, bulk dispenser, BioTek plate washer, and ImageXpress (figures 3, 4)
  • CSC trained users include Tori Klein, and Josh Schwochert. After earning her PhD with this project, Tori Klein accepted a postdoctoral fellowship at the NIH. Josh Schwochert went on to co-found the company, Unnatural Products, which is involved in chemical screening for therapeutic synthetic cyclic peptides. 
  • Former CSC manager, Walter Bray is a coauthor.

a heatmap of clustered compound signatures

58. Lam HN, Lau T, Lentz A, Sherry J, Cabrera-Cortez A, Hug K, Lalljie A, Engel J, Lokey RS, Auerbuch V. Developing Cyclic Peptomers as Broad-Spectrum Type III Secretion System Inhibitors in Gram-Negative Bacteria. Antimicrob Agents Chemother. (2021) Jun 17;65(7):e0169020. doi: 10.1128/AAC.01690-20. Epub 2021 Jun 17. PMID: 33875435 .

  • Absorbance for bacterial growth on CSC EnVision plate reader (figure 3)
  • Cytological profiling (CSC Janus robot, bulk dispenser, BioTek plate washer, and ImageXpress – figure S8)
  • CSC trained users include first author Hanh N Lam, Tannia Lau, Adam Lentz, Karen Hug, and Annalyse Lalljie. After completing her postdoctoral training with this project, Hanh accepted a position as Assistant Professor at Virginia Tech.

cytological profiling images for HeLa cells treated with compounds

57. Chavan AG, Swan JA, Heisler J, Sancar C, Ernst DC, Fang M, Palacios JG, Spangler RK, Bagshaw CR, Tripathi S, Crosby P, Golden SS, Partch CL, LiWang A. Reconstitution of an intact clock reveals mechanisms of circadian timekeeping. Science. (2021) Oct 8;374(6564):eabd4453. doi: 10.1126/science.abd4453. Epub 2021 Oct 8. PMID: 34618577.

  • High throughput liquid handling (CSC Janus robot)
  • Plate reader (CSC EnVision)
  • Featured in Rust, M.J. (2021) Biological rhythms: The suspended animation clock. Curr Biol 31: R1513-R1534.
  • CSC trained users include Jeff Swan, Becca Spangler, and Priya Crosby.

wordcloud with bog words lokey and braymade with free wordcloud generator using entries 1-53 below

56. Parico GCG, Perez I, Fribourgh JL, Hernandez BN, Lee HW, Partch CL. The human CRY1 tail controls circadian timing by regulating its association with CLOCK:BMAL1. Proc Natl Acad Sci U S A. (2020) Nov 10;117(45):27971-27979. doi: 10.1073/pnas.1920653117. Epub 2020 Oct 26.
PMID: 33106415.

  • High throughput liquid handling (CSC Janus robot)
  • Plate reader (CSC EnVision)

55. Wang Y, Zhang L, Wei Y, Huang W, Li L, Wu AA, Dastur A, Greninger P, Bray WM, Zhang CS, Li M, Lian W, Hu Z, Wang X, Liu G, Yao L, Guh JH, Chen L, Wang HR, Zhou D, Lin SC, Xu Q, Shen Y, Zhang J, Jurica MS, Benes CH, Deng X. Pharmacological Targeting of Vacuolar H+-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. (2020) Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9. PMID: 32649904.

  • Cytological profiling (CSC Janus robot, bulk dispenser, BioTek plate washer, and ImageXpress – figure 2)

the chemical structure of VE-P and microscopy images of localization of the click reaction to the lysosome

54. Shi Y, Bray W, Smith AJ, Zhou W, Lagisetti C, Sambucetti L, Crews P, Calaoagan J, Webb T, Lokey RS. An Exon Skipping Screen Identifies Antitumor Drugs That Are Potent Modulators of Pre-mRNA Splicing, Suggesting New Therapeutic Applications. PLoS One. (2020) 15(5):e0233672. doi: 10.1371/journal.pone.0233672. PMID: 32469945. PMCID: PMC7259758.


figure 1 from the paper, a graph showing the Z scores of hits in the assay

53. Fribourgh JL, Srivastava A, Sandate CR, Michael AK, Hsu PL, Rakers C, Nguyen LT, Torgrimson MR, Parico GCG, Tripathi S, Zheng N, Lander GC, Hirota T, Tama F, Partch CL. Dynamics at the serine loop underlie differential affinity of cryptochromes for CLOCK:BMAL1 to control circadian timing. Elife. (2020) Feb 26;9:e55275. doi: 10.7554/eLife.55275. PMID: 32101164.

  • High throughput liquid handling (CSC Janus robot)
  • Plate reader (CSC EnVision)

52. Ono S, Naylor MR, Townsend CE, Okumura C, Okada O, Lokey RS. Conformation and Permeability: Cyclic Hexapeptide Diastereomers. Journal of Chemical Information and Modeling. (2019) 59, 2952-63 Epub 2019/05/03. doi: 10.1021/acs.jcim.9b00217. PubMed PMID: 31042375.

51. Lau TA, Bray WM, Lokey RS. Macrophage Cytological Profiling and Anti-Inflammatory Drug Discovery. Assay and Drug Development Technologies. (2019) 17, 14-6 Epub 2019/01/19. doi: 10.1089/adt.2018.894. PubMed PMID: 30657701; PMCID: PMC6352509.

50. Naylor MR, Ly AM, Handford MJ, Ramos DP, Pye CR, Furukawa A, Klein VG, Noland RP, Edmondson Q, Turmon AC, Hewitt WM, Schwochert J, Townsend CE, Kelly CN, Blanco MJ, Lokey RS. Lipophilic Permeability Efficiency Reconciles the Opposing Roles of Lipophilicity in Membrane Permeability and Aqueous Solubility. J Med Chem. (2018) 61, 11169-82 Epub 2018/11/06. doi: 10.1021/acs.jmedchem.8b01259. PubMed PMID: 30395703.

49. Zhang H, Crews P, Tenney K, Valeriote FA. Cytotoxic Phyllactone Analogs from the Marine Sponge Phyllospongia papyrecea. Med Chem. (2017) 13, 295-300 Epub 2016/10/18. doi: 10.2174/1573406412666161007150828. PubMed PMID: 27748180; PMCID: PMC5384874.
an image of sponge and a chemical structure of the compound of interest

48. White PM, Serbus LR, Debec A, Codina A, Bray W, Guichet A, Lokey RS, Sullivan W. Reliance of Wolbachia on High Rates of Host Proteolysis Revealed by a Genome-Wide RNAi Screen of Drosophila Cells. Genetics. (2017) 205, 1473-88Epub 2017/02/06. doi: 10.1534/genetics.116.198903. PubMed PMID: 28159754; PMCID: PMC5378107.
a diagram of the workflow from image to masks

47. Morgan JM, Duncan MC, Johnson KS, Diepold A, Lam H, Dupzyk AJ, Martin LR, Wong WR, Armitage JP, Linington RG, Auerbuch V. Piericidin A1 Blocks Yersinia Ysc Type III Secretion System Needle Assembly. mSphere. (2017) 2, Epub 2017/02/22. doi: 10.1128/mSphere.00030-17. PubMed PMID: 28217742; PMCID: PMC5311113.

46. McCauley EP, Lam H, Lorig-Roach N, Luu J, Lloyd C, Tenney K, Pietraszkiewicz H, Diaz C, Valeriote FA, Auerbuch V, Crews P. Investigation of the Physical and Bioactive Properties of Bromo- and Iodo- Containing Sponge-Derived Compounds Possessing an Oxyphenylethanamine Core. J Nat Prod. (2017) 80, 3255-66Epub 2017/11/17. doi: 10.1021/acs.jnatprod.7b00694. PubMed PMID: 29144750.
a picture of a sea sponge with a diagram showing how fractions were selected with appropriate activity

45. Martucci H, Campit SE, Gee SR, Bray WM, Gokey T, Cada AK, Yen TY, Minoura K, Guliaev AB, Lokey RS, Amagata T. Naphthablins B and C, Meroterpenoids Identified from the Marine Sediment- Derived Streptomyces sp. CP26-58 Using HeLa Cell-Based Cytological Profiling. J Nat Prod. (2017) 80, 684-91, Epub 2017/01/28. doi: 10.1021/acs.jnatprod.6b00996. PubMed PMID: 28128950.
an agar plate with colonies, and structures of two hit compounds

44. Lam H, Schwochert J, Lao Y, Lau T, Lloyd C, Luu J, Kooner O, Morgan J, Lokey S, Auerbuch V. Synthetic Cyclic Peptomers as Type III Secretion System Inhibitors. Antimicrob Agents Chemother. (2017) 61, Epub 2017/06/28. doi: 10.1128/AAC.00060-17. PubMed PMID: 28652236; PMCID: PMC5571333.
microscopy images of cells

43. Suresh S, Schlecht U, Xu W, Bray W, Miranda M, Davis RW, Nislow C, Giaever G, Lokey RS, St Onge RP. Systematic Mapping of Chemical-Genetic Interactions in Saccharomyces cerevisiae. Cold Spring Harbor protocols. (2016) 2016, Epub 2016/09/03. doi: 10.1101/pdb.top077701. PubMed PMID: 27587783.
a heatmap showing different phenotypes

42. Sanchez LM, Cheng AT, Warner CJ, Townsley L, Peach KC, Navarro G, Shikuma NJ, Bray WM, Riener RM, Yildiz FH, Linington RG. Biofilm Formation and Detachment in Gram-Negative Pathogens Is Modulated by Select Bile Acids. PLoS One. (2016) 11, e0149603, Epub 2016/03/19. doi: 10.1371/journal.pone.0149603. PubMed PMID: 26992172; PMCID: PMC4798295.

41. Pye CR, Bray WM, Brown ER, Burke JR, Lokey RS, Rubin SM. A Strategy for Direct Chemical Activation of the Retinoblastoma Protein. ACS Chem Biol. (2016) 11, 1192-7, Epub 2016/02/05. doi: 10.1021/acschembio.6b00011. PubMed PMID: 26845289; PMCID: PMC5117131.

40. Giltrap AM, Dowman LJ, Nagalingam G, Ochoa JL, Linington RG, Britton WJ, Payne RJ. Total Synthesis of Teixobactin. Org Lett. (2016) 18, 2788-91, Epub 2016/05/19. doi: 10.1021/acs.orglett.6b01324. PubMed PMID: 27191730.

39. Bray W, Lokey RS. A High-Throughput Yeast Halo Assay for Bioactive Compounds. Cold Spring Harbor protocols. (2016) 2016, Epub 2016/09/03. doi: 10.1101/pdb.prot088047. PubMed PMID: 27587777.

38. Warner CJ, Cheng AT, Yildiz FH, Linington RG. Development of benzo[1,4]oxazines as biofilm inhibitors and dispersal agents against Vibrio cholerae. Chem Commun (Camb). (2015) 51, 1305-8, Epub 2014/12/06. doi: 10.1039/c4cc07003h. PubMed PMID: 25479128; PMCID: PMC4288701.

37. Theodore CM, Lorig-Roach N, Still PC, Johnson TA, Draskovic M, Schwochert JA, Naphen CN, Crews MS, Barker SA, Valeriote FA, Lokey RS, Crews P. Biosynthetic products from a nearshore-derived gram- negative bacterium enable reassessment of the kailuin depsipeptides. J Nat Prod. (2015) 78, 441-52, Epub 2015/02/24. doi: 10.1021/np500840n. PubMed PMID: 25699470; PMCID: PMC4699550.

36. Ochoa JL, Bray WM, Lokey RS, Linington RG. Phenotype-Guided Natural Products Discovery Using Cytological Profiling. J Nat Prod. (2015) 78, 2242-8, Epub 2015/08/22. doi: 10.1021/acs.jnatprod.5b00455. PubMed PMID: 26292657.

35. Leon B, Navarro G, Dickey BJ, Stepan G, Tsai A, Jones GS, Morales ME, Barnes T, Ahmadyar S, Tsiang M, Geleziunas R, Cihlar T, Pagratis N, Tian Y, Yu H, Linington RG. Abyssomicin 2 reactivates latent HIV-1 by a PKC- and HDAC-independent mechanism. Org Lett. (2015) 17, 262-5, Epub 2015/01/07. doi: 10.1021/ol503349y. PubMed PMID: 25560385; PMCID: PMC5755699.

34. Leon B, Haeckl FP, Linington RG. Optimized quinoline amino alcohols as disruptors and dispersal agents of Vibrio cholerae biofilms. Organic & Biomolecular Chemistry. (2015) 13, 8495-9, Epub 2015/07/15. doi: 10.1039/c5ob01134e. PubMed PMID: 26156292; PMCID: PMC4521388.

33. Kurita KL, Glassey E, Linington RG. Integration of high-content screening and untargeted metabolomics for comprehensive functional annotation of natural product libraries. Proceedings of the National Academy of Sciences of the United States of America. (2015) 112, 11999-2004, Epub 2015/09/16. doi: 10.1073/pnas.1507743112. PubMed PMID: 26371303; PMCID: PMC4593099.

32. Effenberger KA, James RC, Urabe VK, Dickey BJ, Linington RG, Jurica MS. The Natural Product N- Palmitoyl-l-leucine Selectively Inhibits Late Assembly of Human Spliceosomes. J Biol Chem. (2015) 290, 27524-31, Epub 2015/09/27. doi: 10.1074/jbc.M115.673210. PubMed PMID: 26408199; PMCID: PMC4646004.

31. Wride DA, Pourmand N, Bray WM, Kosarchuk JJ, Nisam SC, Quan TK, Berkeley RF, Katzman S, Hartzog GA, Dobkin CE, Scott Lokey R. Confirmation of the cellular targets of benomyl and rapamycin using next- generation sequencing of resistant mutants in S. cerevisiae. Mol Biosyst. (2014) 10, 3179-87, Epub 2014/09/27. doi: 10.1039/c4mb00146j. PubMed PMID: 25257345; PMCID: PMC4653042.

30. Schulze CJ, Bray WM, Loganzo F, Lam MH, Szal T, Villalobos A, Koehn FE, Linington RG. Borrelidin B: isolation, biological activity, and implications for nitrile biosynthesis. J Nat Prod. (2014) 77, 2570-4, Epub 2014/11/14. doi: 10.1021/np500727g. PubMed PMID: 25393949.

29. Navarro G, Cheng AT, Peach KC, Bray WM, Bernan VS, Yildiz FH, Linington RG. Image-based 384- well high-throughput screening method for the discovery of skyllamycins A to C as biofilm inhibitors and inducers of biofilm detachment in Pseudomonas aeruginosa. Antimicrob Agents Chemother. (2014) 58, 1092- 9, Epub 2013/12/04. doi: 10.1128/AAC.01781-13. PubMed PMID: 24295976; PMCID: PMC3910817.

28. Effenberger KA, Anderson DD, Bray WM, Prichard BE, Ma N, Adams MS, Ghosh AK, Jurica MS. Coherence between cellular responses and in vitro splicing inhibition for the anti-tumor drug pladienolide B and its analogs. J Biol Chem. (2014) 289, 1938-47, Epub 2013/12/05. doi: 10.1074/jbc.M113.515536. PubMed PMID: 24302718; PMCID: PMC3900944.

27. Duncan MC, Wong WR, Dupzyk AJ, Bray WM, Linington RG, Auerbuch V. An NF-kappaB-based high- throughput screen identifies piericidins as inhibitors of the Yersinia pseudotuberculosis type III secretion system. Antimicrob Agents Chemother. (2014) 58, 1118-26, Epub 2013/12/04. doi: 10.1128/aac.02025-13. PubMed PMID: 24295981; PMCID: PMC3910828.

26. Yang JY, Sanchez LM, Rath CM, Liu X, Boudreau PD, Bruns N, Glukhov E, Wodtke A, de Felicio R, Fenner A, Wong WR, Linington RG, Zhang L, Debonsi HM, Gerwick WH, Dorrestein PC. Molecular networking as a dereplication strategy. J Nat Prod. (2013) 76, 1686-99, Epub 2013/09/13. doi: 10.1021/np400413s. PubMed PMID: 24025162; PMCID: PMC3936340.

25. Woehrmann MH, Bray WM, Durbin JK, Nisam SC, Michael AK, Glassey E, Stuart JM, Lokey RS. Large-scale cytological profiling for functional analysis of bioactive compounds. Mol Biosyst. (2013) 9, 2604- 17, Epub 2013/09/24. doi: 10.1039/c3mb70245f. PubMed PMID: 24056581.

24. Schulze CJ, Bray WM, Woerhmann MH, Stuart J, Lokey RS, Linington RG. "Function-first" lead discovery: mode of action profiling of natural product libraries using image-based screening. Chemistry & Biology. (2013) 20, 285-95, Epub 2013/02/27. doi: 10.1016/j.chembiol.2012.12.007. PubMed PMID: 23438757; PMCID: PMC3584419.

23. Peach KC, Cheng AT, Oliver AG, Yildiz FH, Linington RG. Discovery and biological characterization of the auromomycin chromophore as an inhibitor of biofilm formation in Vibrio cholerae. Chembiochem. (2013) 14, 2209-15, Epub 2013/10/10. doi: 10.1002/cbic.201300131. PubMed PMID: 24106077; PMCID: PMC3947468.

22. Peach KC, Bray WM, Winslow D, Linington PF, Linington RG. Mechanism of action-based classification of antibiotics using high-content bacterial image analysis. Mol Biosyst. (2013) 9, 1837-48, Epub 2013/04/24. doi: 10.1039/c3mb70027e. PubMed PMID: 23609915; PMCID: PMC3674180.

21. Leon B, Fong JC, Peach KC, Wong WR, Yildiz FH, Linington RG. Development of quinoline-based disruptors of biofilm formation against Vibrio cholerae. Org Lett. (2013) 15, 1234-7, Epub 2013/03/07. doi: 10.1021/ol400150z. PubMed PMID: 23461487.

20. Kwuan L, Adams W, Auerbuch V. Impact of host membrane pore formation by the Yersinia pseudotuberculosis type III secretion system on the macrophage innate immune response. Infection and Immunity. (2013) 81, 905-14, Epub 2013/01/09. doi: 10.1128/iai.01014-12. PubMed PMID: 23297383; PMCID: PMC3584892.

19. Effenberger KA, Perriman RJ, Bray WM, Lokey RS, Ares M, Jr., Jurica MS. A high-throughput splicing assay identifies new classes of inhibitors of human and yeast spliceosomes. J Biomol Screen. (2013) 18, 1110-20, Epub 2013/06/19. doi: 10.1177/1087057113493117. PubMed PMID: 23771823; PMCID: PMC3902173.

18. Zuckerman NB, Myers AS, Quan TK, Bray WM, Lokey RS, Hartzog GA, Konopelski JP. Structural determination of NSC 670224, synthesis of analogues and biological evaluation. ChemMedChem. (2012) 7, 761-5, Epub 2012/03/02. doi: 10.1002/cmdc.201200038. PubMed PMID: 22378491; PMCID: PMC3516922.

17. Serbus LR, Landmann F, Bray WM, White PM, Ruybal J, Lokey RS, Debec A, Sullivan W. A cell-based screen reveals that the albendazole metabolite, albendazole sulfone, targets Wolbachia. PLoS pathogens. (2012) 8, e1002922, Epub 2012/10/03. doi: 10.1371/journal.ppat.1002922. PubMed PMID: 23028321; PMCID: PMC3447747.

16. Sanchez LM, Wong WR, Riener RM, Schulze CJ, Linington RG. Examining the fish microbiome: vertebrate-derived bacteria as an environmental niche for the discovery of unique marine natural products. PLoS One. (2012) 7, e35398, Epub 2012/05/11. doi: 10.1371/journal.pone.0035398. PubMed PMID: 22574119; PMCID: PMC3344833.

15. Navarro G, Chokpaiboon S, De Muylder G, Bray WM, Nisam SC, McKerrow JH, Pudhom K, Linington RG. Hit-to-lead development of the chamigrane endoperoxide merulin A for the treatment of African sleeping sickness. PLoS One. (2012) 7, e46172, Epub 2012/10/03. doi: 10.1371/journal.pone.0046172. PubMed PMID: 23029428; PMCID: PMC3459870.

14. Watts KR, Morinaka BI, Amagata T, Robinson SJ, Tenney K, Bray WM, Gassner NC, Lokey RS, Media J, Valeriote FA, Crews P. Biostructural features of additional jasplakinolide (jaspamide) analogues. J Nat Prod. (2011) 74, 341-51, Epub 2011/01/19. doi: 10.1021/np100721g. PubMed PMID: 21241058; PMCID: PMC3070360.

13. Tamble CM, St Onge RP, Giaever G, Nislow C, Williams AG, Stuart JM, Lokey RS. The synthetic genetic interaction network reveals small molecules that target specific pathways in Sacchromyces cerevisiae. Mol Biosyst. (2011) 7, 2019-30, Epub 2011/04/14. doi: 10.1039/c0mb00298d. PubMed PMID: 21487606; PMCID: PMC4653045.

12. Peach KC, Bray WM, Shikuma NJ, Gassner NC, Lokey RS, Yildiz FH, Linington RG. An image-based 384-well high-throughput screening method for the discovery of biofilm inhibitors in Vibrio cholerae. Mol Biosyst. (2011) 7, 1176-84, Epub 2011/01/20. doi: 10.1039/c0mb00276c. PubMed PMID: 21246108.

11. Johnson TA, Sohn J, Inman WD, Estee SA, Loveridge ST, Vervoort HC, Tenney K, Liu J, Ang KK, Ratnam J, Bray WM, Gassner NC, Shen YY, Lokey RS, McKerrow JH, Boundy-Mills K, Nukanto A, Kanti A, Julistiono H, Kardono LB, Bjeldanes LF, Crews P. Natural product libraries to accelerate the high-throughput discovery of therapeutic leads. J Nat Prod. (2011) 74, 2545-55, Epub 2011/12/02. doi: 10.1021/np200673b. PubMed PMID: 22129061; PMCID: PMC3246535.

10. Woehrmann MH, Gassner NC, Bray WM, Stuart JM, Lokey S. HALO384: a halo-based potency prediction algorithm for high-throughput detection of antimicrobial agents. J Biomol Screen. (2010) 15, 196- 205, Epub 2010/01/21. doi: 10.1177/1087057109355060. PubMed PMID: 20086209.

9. Robinson SJ, Morinaka BI, Amagata T, Tenney K, Bray WM, Gassner NC, Lokey RS, Crews P. New structures and bioactivity properties of jasplakinolide (jaspamide) analogues from marine sponges. J Med Chem. (2010) 53, 1651-61, Epub 2010/02/04. doi: 10.1021/jm9013554. PubMed PMID: 20121114; PMCID: PMC2848536.

8. Liu X, Beyhan S, Lim B, Linington RG, Yildiz FH. Identification and characterization of a phosphodiesterase that inversely regulates motility and biofilm formation in Vibrio cholerae. J Bacteriol. (2010) 192, 4541-52, Epub 2010/07/14. doi: 10.1128/JB.00209-10. PubMed PMID: 20622061; PMCID: PMC2937418.

7. Kapitzky L, Beltrao P, Berens TJ, Gassner N, Zhou C, Wuster A, Wu J, Babu MM, Elledge SJ, Toczyski D, Lokey RS, Krogan NJ. Cross-species chemogenomic profiling reveals evolutionarily conserved drug mode of action. Molecular Systems Biology. (2010) 6, 451, Epub 2010/12/24. doi: 10.1038/msb.2010.107. PubMed PMID: 21179023; PMCID: PMC3018166.

6. Inman WD, Bray WM, Gassner NC, Lokey RS, Tenney K, Shen YY, Tendyke K, Suh T, Crews P. A beta-carboline alkaloid from the Papua New Guinea marine sponge hyrtios reticulatus. J Nat Prod. (2010) 73, 255-7, Epub 2009/12/17. doi: 10.1021/np9005426. PubMed PMID: 20000782; PMCID: PMC2829331.

5. Seballos L, Richards N, Stevens DJ, Patel M, Kapitzky L, Lokey S, Millhauser G, Zhang JZ. Competitive binding effects on surface-enhanced Raman scattering of peptide molecules. Chem Phys Lett. (2007) 447, 335-9, Epub 2007/10/01. doi: 10.1016/j.cplett.2007.09.040. PubMed PMID: 20648229; PMCID: PMC2905820.

4. Ralifo P, Sanchez L, Gassner NC, Tenney K, Lokey RS, Holman TR, Valeriote FA, Crews P. Pyrroloacridine alkaloids from Plakortis quasiamphiaster: structures and bioactivity. J Nat Prod. (2007) 70, 95- 9, Epub 2007/01/27. doi: 10.1021/np060585w. PubMed PMID: 17253856; PMCID: PMC1839848.

3. Gassner NC, Tamble CM, Bock JE, Cotton N, White KN, Tenney K, St Onge RP, Proctor MJ, Giaever G, Nislow C, Davis RW, Crews P, Holman TR, Lokey RS. Accelerating the discovery of biologically active small molecules using a high-throughput yeast halo assay. J Nat Prod. (2007) 70, 383-90, Epub 2007/02/13. doi: 10.1021/np060555t. PubMed PMID: 17291044; PMCID: PMC2533267.

2. Deschamps JD, Gautschi JT, Whitman S, Johnson TA, Gassner NC, Crews P, Holman TR. Discovery of platelet-type 12-human lipoxygenase selective inhibitors by high-throughput screening of structurally diverse libraries. Bioorg Med Chem. (2007) 15, 6900-8, Epub 2007/09/11. doi: 10.1016/j.bmc.2007.08.015. PubMed PMID: 17826100; PMCID: PMC2203963.

1. Boot CM, Gassner NC, Compton JE, Tenney K, Tamble CM, Lokey RS, Holman TR, Crews P. Pinpointing pseurotins from a marine-derived Aspergillus as tools for chemical genetics using a synthetic lethality yeast screen. J Nat Prod. (2007) 70, 1672-5, Epub 2007/10/13. doi: 10.1021/np070307c. PubMed PMID: 17929896.