~~~can’t find something?~~~please note that this information is being migrated to the instrument pages, linked on the “Equipment & Resources” page~~~under construction~~~~thanks!~~~
Here is a collection of our current instrument instructions, assay protocols, suggested methodology, and training resources for experiments conducted with the CSC.
Link describing the expansion of these resources in 2021-2022.
For any Google documents linked here, if not already publicly available, please ask CSC personnel for permission to view, and thereafter you will be able to access them from the links below while you are logged in to your Google account.
To locate hard copies of instrument manuals and documentation, please see the wall shelf in the CSC lab space above the general lab bench.
Compound Libraries
- Inventory of our microplates stored in the freezer incl nomenclature, locations here
Plate Reader
» VIDEO! of our plate reader in action
» VIDEO! of our plate reader virtual group training with Bill Ortiz from Perkin Elmer on February 17, 2022
- Mammalian cell viability drug screen by Cell Titer Glo in 384 well format
- A presentation on TR-FRET and alpha screen (by Beverley of the CSC for the Rubin lab meeting May 2022)
- Perkin Elmer information on alpha screen here
Imagers
–ImageXpress–
» VIDEO! of our ImageXpress in action
» VIDEO! of MetaXpress analysis and export
- Immuno-cytological profiling in RAW cells protocol and another writeup here
- ImmunoCP measurements – historical and current
- Cytological profiling in HeLa cells protocol
- A table to help schedule CP experiments
Dispensing
» VIDEO! Of our Matrix WellMate in action
- Multidrop cassette calibration
- Pinning robot calibration
- Thermo Fisher Scientific technical note TN-ALH-MDcombi01-0209 with lots of tips on dispensing cells, frothing, clogging, etc.
- Thermo Fisher MultiDrop Customer Corner
- Click on Reagent Atlas for list of compatible solutions
- Click on Maintenance for instructions on operating and maintaining the cassettes and device
- There are further useful bits on this site including FAQs.
- Thermo Scientific technical note COL114200 0421 about reproducibility of live cell dispensing in a MultiDrop. The CSC recommends to constantly mix the cell suspension to ensure even plating.
- Thermo Scientific product note about life time expectancy of MultiDrop cassettes
- Thermo Scientific product tips about clogs in MultiDrop
Plate handling
» VIDEO! of our PlateLoc plate sealer in action
» VIDEO! of the Bio-Stak and plate washer in action
» VIDEO! of this plate washer in action
